Correction: Exocytosis of ATP From Astrocytes Modulates Phasic and Tonic Inhibition in the Neocortex

1. Lalo U, Palygin O, Rasooli-Nejad S, Andrew J, Haydon PG, et al. (2014) Exocytosis of ATP From Astrocytes Modulates Phasic and Tonic Inhibition in the Neocortex. PLoS Biol 12(1): e1001747. doi:10.1371/journal.pbio.1001747 Figure 2. Ca2+-dependent release of ATP can be evoked in the cortical astrocytes of the wild-type but not of the dn-SNARE mice. Release of ATP from cortical astrocytes of wild-type (A, B) and dnSNARE mice (C) was detected using the sniffer cells as described in Figure 1. (A) Elevation of cytosolic Ca2+ level was elicited in the astrocytes by UV uncaging and by rapid application of the agonist of PAR-1 metabotropic receptor TFLLR (10 mM) or the agonist of glutamate ionotropic receptor NMDA (20 mM). (B) Inhibition of vacuolar H-ATPase in the astrocytes with Bafilomycin A1 (1 mM for 2 h) dramatically decreased both the amplitude and frequency of phasic currents. (C) Elevation of the Ca2+ level in any of the dn-SNARE astrocytes did not lead to activation of phasic purinergic currents in the sniffer cell. Inlays in (A–C) show examples of individual phasic currents recorded at moments indicated; scale bars are 50 ms and 10 pA. (D) The amplitude and decay time distributions of purinergic currents recorded in the HEK293-P2X2 cells after stimulation of the astrocytes; data were pooled for number of experiments indicated in (E). The grey dotted line shows the best fit of quantal model to the distribution of UV-activated currents. (E) The pooled data (mean 6 SD for indicated numbers of experiments) on net release of ATP were assessed as total charge transferred by spontaneous currents in the sniffer cell. The effects of bafylomicin and dn-SNARE expression on net charge transferred by purinergic currents were statistically significant at p = 0.005 (twopopulation t test). doi:10.1371/journal.pbio.1001747.g002 Citation: The PLOS Biology Staff (2014) Correction: Exocytosis of ATP From Astrocytes Modulates Phasic and Tonic Inhibition in the Neocortex. PLoS Biol 12(4): e1001857. doi:10.1371/journal.pbio.1001857